提供ELISA試劑盒/上海ELISA試劑盒價(jià)格/上海小鼠血栓素B2ELISA試劑盒價(jià)格
小鼠血栓素B2(TXB2)酶聯(lián)免疫分析(ELISA)試劑盒
實(shí)驗(yàn)?zāi)康模罕驹噭┖袃H供研究使用,用于測(cè)定小鼠血清,血漿及相關(guān)液體樣本中血栓素B2(TXB2)的含量。
實(shí)驗(yàn)原理:本試劑盒應(yīng)用雙抗體夾心法測(cè)定標(biāo)本中小鼠血栓素B2(TXB2)水平。用純化的小鼠血栓素B2(TXB2)抗體包被微孔板,制成固相抗體,往包被單抗的微孔中依次加入血栓素B2(TXB2),再與HRP標(biāo)記的血栓素B2(TXB2)抗體結(jié)合,形成抗體-抗原-酶標(biāo)抗體復(fù)合物,經(jīng)過(guò)徹底洗滌后加底物TMB顯色。TMB在HRP酶的催化下轉(zhuǎn)化成藍(lán)色,并在酸的作用下轉(zhuǎn)化成最終的黃色。顏色的深淺和樣品中的血栓素B2(TXB2)呈正相關(guān)。用酶標(biāo)儀在450nm波長(zhǎng)下測(cè)定吸光度(OD值),通過(guò)標(biāo)準(zhǔn)曲線計(jì)算樣品中小鼠血栓素B2(TXB2)濃度。
Drug Names
Generic Name:Mouse Thromboxane B2 (TXB2) ELISA Kit.
Purpose
This kit allows for the determination of TXB2 concentrations in Mouse serum, blood plasma, and other biological fluids.
Principle of the assay
The kit assay Mouse TXB2 level in the sample,use Purified Mouse TXB2 antibody to coat microtiter plate wells, make solid-phase antibody, then add TXB2 to wells, Combined TXB2 antibody which With HRP labeled, become antibody - antigen - enzyme-antibody complex, after washing Completely, Add TMB substrate solution,TMB substrate becomes blue color At HRP enzyme-catalyzed, reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. The concentration of TXB2 in the samples is then determined by comparing the O.D. of the samples to the standard curve.
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上海華壹生物科技有限公司
Shanghai HuaYi Bio-technology Co.,Ltd
.華壹生物科技///提供ELISA試劑盒/上海ELISA試劑盒價(jià)格/上海小鼠血栓素B2ELISA試劑盒價(jià)格 |